Water movement and variation in discharge are important structuring features for aquatic communities. Everglades restoration includes increasing flow rates (from < 0.5 to 1-3 cm/s) in a large (~6,000 km2) wetland landscape, but the net effects of discharge variation for the food web have not been examined. We conducted a 6-week in situ enclosure experiments in a series of 3-4 sloughs that experienced different levels of experimental discharge during the dry season of 2018 and in the wet seasons of 2018, 2019, 2020. Mesh field enclosures were placed perpendicular to water flow and natural periphyton mats and small (1-2 cm length) consumers (mostly fish) were added to each. At the end of the experiment, food web components (biofilm on standardized surfaces, flocculent organic matter, periphyton, and consumers) were collected and analyzed for stoichiometric ratios (C:N:P) and stable isotopes (δ13C, δ15N). For the latter two experimental runs, consumers were measured and weighed, and the fish stomachs were dissected for gut contents. We conducted linear models to test for variation in mean stoichiometric ratios, trophic positions and biomasses of consumers between the sites receiving the most discharge compared to the two sites receiving low/no discharge. Periphyton and biofilm at the two high-flow sites had the lowest ratios of C:P and N:P; higher food quality was associated with more flow. Consumer body composition exhibited strict stoichiometric homeostasis, but omnivorous taxa decreased their trophic position in the high discharge sites. While animals at the highest discharge site generally accrued more biomass, the biomass response was only significant for all three taxa (sailfin mollies, grass shrimp and eastern mosquitofish) in one study year (2019). Our results suggest that flow restoration can impact bottom-up energy flow by improving food quality of basal resources and alter food-web functions.