Southern Appalachian rivers and streams host some of the highest diversity of freshwater fish of any temperate region worldwide, with many endemic species restricted to small ranges. Despite the high levels of diversity and endemism of fish in this region, the physiology of many of these species and how they respond to varying environmental conditions is understudied. In this study, we seek to quantify the somatic growth of two non-game species of benthic invertivores, Greenfin Darters (Nothonotus chlorobranchius) and (Redline Darters (Nothonotus rufilineatus), under different temperature and feeding conditions. To achieve this, we performed a 12-week laboratory growth experiment on captive-bred individuals from wild-caught populations. We kept year-1 individuals for each species at three different temperature treatments, 14, 20, and 26 degrees Celsius, corresponding to different potential climate change scenarios. For each temperature treatment, half the tanks received a maximum feeding treatment at each temperature treatment and a 50% feeding treatment. Fish were photographed at the start of the experiment, then bi-weekly until the end of the 12-week experimental period. Pictures were then analyzed using Image J to measure the length of each fish throughout the experiment. Preliminary data analysis indicates that both temperature and feeding treatment significantly impacted the growth of these species, with both species exhibiting greater growth under the maximum feeding treatment and growth differing depending on the temperature treatment. These findings give insight into how these species may perform under different climate change scenarios and broaden our understanding of the basic biology of these understudied species. This information can help inform effective conservation efforts for these and other closely related species.