Native fish species in the U.S. Caribbean, Puerto Rico (PR) and U.S. Virgin Islands (USVI), are under threat from the loss of habitat from development, introduction of invasive species and the increasing occurrence of droughts and other effects of a rapidly changing climate in the Caribbean. All native freshwater fishes in the region are diadromous and depend on aquatic connectivity to complete their lifecycles. Manmade barriers (e.g., dams and culverts) can have negative impacts on the populations of migratory fishes. The USFWS Caribbean Ecological Services Field Office (CESFO) in partnership with the USFWS Fish and Aquatic Conservation Program has collaborated with PR and USVI state fish and wildlife agencies, universities, local NGOs and the Southeast Aquatic Resources Partnership (SARP) to identify and prioritize aquatic migration barriers across the region. Hundreds of road crossings and dams have currently been assessed and are included in the National Aquatic Barrier Inventory and Prioritization tool. In Puerto Rico, in 2019, the removal of the Cambalache Dam in the Rio Grande watershed of Arecibo was successful in reconnecting 25km of freshwater habitat for species of greatest conservation need including Sirajo gobies and American eels and there are future plans for further habitat restoration projects within other critical watersheds. In the USVI, work has only recently begun since 2023 and there are current efforts in completing stream barrier assessments across the three major islands (e.g., St. Croix, St. Thomas and St. John) along with conducting surveys for freshwater species of greatest conservation need. This presentation will highlight the continued plans that CESFO has to work with its federal and local partners in achieving fish and aquatic conservation program objectives across the U.S. Caribbean and improving the management of freshwater fauna for the benefit of the islands’ wildlife, local residents and visitors alike. Â