The southern United States supports a disproportionately high number of native fishes, along with one of the highest number of imperiled fishes in need of conservation action. Local endemic species are often particularly vulnerable, so understanding their life history and status is paramount to their effective management. The Etowah Bridled Darter, Percina freemanorum, is a newly described fish species endemic to the Upper Etowah River in Georgia, known from approximately 50 km of river. Our objectives are to evaluate P. freemanorum’s microhabitat associations, including substrate, depth, velocity, and watershed characteristics such as stream order, and current and past land use. We conducted summer snorkel surveys in the Upper Etowah River basin, recording microhabitat conditions at each location where an individual was seen, and we used these data to augment a long-term fish monitoring dataset. We modeled watershed characteristics at sites with and without P. freemanorum to determine the species’ landscape scale preferences. Our initial microhabitat results indicate P. freemanorum prefers an average depth of 0.43 meters, average velocity of 0.15 m/s, over sand, gravel, and cobble. Initial landscape scale results indicate that P. freemanorum is negatively associated with the percent of upstream area that was historically agriculture and positively associated with the percent of upstream area that is currently forested. These results will be useful for managing P. freemanorum populations in the Upper Etowah River, and may serve as a template for using assessments at multiple scales to better understand species’ habitat requirements.