In recent years, Hawaii has been inundated with introduced species, many of which have become disruptive to local ecosystems. In 2018, water flow was restored to a series of desiccated streams in East Maui, Hawaii after almost two centuries of diverted flow. Since the return of historic flow to the stream systems in East Maui, little research has been performed to determine the presence of native or invasive aquatic species. Invasive species displace native and endemic species with economic and cultural significance to the local Hawaiian communities. Many Hawaiian endemic species have a unique life cycle, in which eggs are laid in mountain streams and larvae are swept out to the ocean after hatching. Larvae then develop into adults before returning to mountain streams. In 2024 my team and I collected eDNA and water quality data using metrics such as temperature, pH, dissolved oxygen, chlorophyll A, phycocyanin, and specific conductance. Using this data, I examined whether the historic diversion and subsequent restoration of natural flow to streams in East Maui has been conducive for the reintroduction of native aquatic species, or if it has left an opening for invasion. I have compiled results into a Species Distribution Map using ArcGIS. Nā Moku ‘Aupuni ‘o Ko’olau Hui (Nā Moku) is a non-profit based in East Maui whose mission is to protect and restore local stream ecosystems according to traditional Hawaiian practices. This data is useful for identifying conditions in which invasive species have been able to establish faster than native species and aids in conservation targeting.