Oral Presentation Society for Freshwater Science 2025 Annual Meeting

Seasonal optical properties of DOM, POM, and BOM in a piedmont watershed in central Alabama, USA (118360)

Zacharie Loveless 1 , Arial Shogren 1 , Stephen Plont 1 , Chelsea Smith 1 , Jonathan Benstead 1 , Carla Atkinson 1
  1. The University of Alabama, AL, United States

Stream OM is a heterogeneous mixture of material, but can be partitioned operationally into dissolved, suspended particulate, and benthic pools (DOM, SPOM, and BOM, respectively). While  the availability and composition of OM affects carbon processing rates, temporal and spatial trends in these characteristics across different OM pools are rarely well resolved  in river networks. To address this knowledge gap, we examined the seasonal and spatial patterns of OM availability and composition in a small, forested stream network: the Pendergrass Creek watershed within the Talladega National Forest in central Alabama, USA. Every three weeks from January 2024 through April 2025, we sampled OM pools at four nested sampling sites, from tributaries to the watershed mainstem. At each site, we quantified optical properties of each OM pool using excitation-emission matrices (EEMs) as a proxy of OM composition. In addition, we deployed a suite of high-frequency water quality sensors (YSI EXO2) and used high-frequency measurements of fluorescent DOM (fDOM) and turbidity to establish relationships between OM concentration, optical properties, and discharge. Our preliminary analyses revealed spatial variation in DOM, SPOM, and BOM composition, where the OM at the most upstream site tended to be more degraded and humic (Average HIX = 0.865), while OM at the downstream sites was less humic (Average HIX = 0.665). Concentrations of fDOM across sites followed seasonal trends, increasing during the leaf-off period and during periods of increased discharge. Our preliminary results indicate that the composition of DOM, SPOM, and BOM pools shifts across space and time, with implications for the fate of carbon in headwater stream networks and the need for future research to examine and consider multiple pools of OM in addition to DOM.