Despite decades of implementing conservation management practices (CMPs) to reduce abiotic and biotic stressors within the Chesapeake Bay Watershed, there has been relatively little improvement in stream biological conditions, including macroinvertebrate diversity, density, and function. The primary stressors, such as elevated specific conductivity and sediment, negatively affect macroinvertebrate respiration and infill interstitial habitats between benthic substrates. To assess the biological condition of streams, bioassessments are performed where sampling often focuses in riffles due to their high biological activity. However, a riffle-only approach could miss taxa only present in other stream hydrogeomorphic zones, such as depositional habitats (leaf packs, debris accumulations, undercut banks, and large woody debris). We quantitatively sampled five depositional habitats and five riffles at 30 stream sites representing a gradient of agricultural land use and implemented CMPs to identify the potential biological and functional responses of macroinvertebrates within riffles and depositional habitats. Within riffles, we observed a range in taxa richness (39 to 53 taxa) and biomass (3704 to 13316 mg/m2). Streams with more upstream pastureland (> 57% in watershed) had approximately 4x greater biomass, and 7x greater density compared to sites with less pastureland (<14%). We will compare alpha, beta, and gamma diversity within microhabitats at our stream sites to assess how different microhabitats affect taxonomic and functional trait diversity. We will also compare biomass and density between riffle and depositional habitats to evaluate if land use and CMP intensity scores are increasing areal depositional habitat area and resulting in biological uplift. We hypothesize that total biomass, density, and taxonomic and functional trait diversity will be greater in watersheds with more CMPs from greater habitat types beyond riffles as microhabitats re-form following CMP implementation. Quantifying how CMPs affect the entire habitat breadth of streams is essential to identify if CMPs result in macroinvertebrate biological uplift.