The invasive benthic invertivore, round goby, was introduced to the Great Lakes in 1990. It has since spread into the Niagara River and many of its tributaries, negatively affecting native aquatic invertebrate and fish communities. Previous studies regarding round goby diet have been taxonomically focused on other invaders such as zebra mussels (Dreissena polymorpha), or more common invertebrate prey such as chironomids. However, an assessment of an opportunistic vs. preferential feeding strategy is a significant gap in our understanding of the full impact of round goby invasion. To assess round goby feeding strategy, we first quantified macroinvertebrate communities along two Niagara River tributaries, Ellicott and Tonawanda Creek. This available prey was compared to the gut content of round goby collected at the same sites. Preliminary macroinvertebrate community data exhibit a majority Chironomidae across all sites, with other high-density macroinvertebrates including Hydropsychidae larvae and Amphipoda. We expect that round goby exhibit preference for larger prey because it is energy efficient. However, at sites with higher abundances and diversity, we expect that round goby may have differences in preference due to an increased choice and availability. If round goby exhibit strong dietary preferences, sites with the appropriate prey bases may be more vulnerable to invasion impacts. If round goby are truly opportunistic feeders, prey base may not be informative in guiding management activities, in which case we can focus monitoring efforts on other factors. By understanding diet preference of the round goby, we can help monitor and manage future invasion.