Bioturbation by chironomid larvae plays a key role in maintaining freshwater ecosystem function. Chironomids ventilate their burrows through constant undulatory movements, pumping oxygenated water from the water column into the sediment, resulting in aerobic conditions around the burrow. This localized increase in oxygen levels influences nutrient cycling and microbial activity in the sediment. In tropical environments, the effects of chironomid burrowing behavior and the impact of rising temperatures on bioturbation processes remain poorly understood. Given climate change projections, evaluating temperature-driven shifts in chironomid activity is crucial for predicting their effects on sediment biogeochemistry. This study examines how temperature influences Chironomus vitellinus ventilation activity and irrigation-mediated benthic fluxes through controlled laboratory experiments simulating tropical base and extreme temperature conditions observed in freshwater environments in Puerto Rico (22°C, 25°C, and 30°C). Larvae were placed in U-shaped artificial burrows made of paper, and their movements were recorded for 20 minutes. Five-minute segments were analyzed using BORIS software to quantify undulation frequency, as well as the frequency, length, and duration of pumping periods (periods of consecutive undulations). Results indicate that undulation frequency increased with temperature, with larvae at 30°C exhibiting significantly more undulations (4,616 und/hr) than those at 22°C (2,810 und/hr) and 25°C (3,566 und/hr). While pumping period frequency remained stable across temperatures, the length of these events was greater at 25°C and 30°C compared to 22°C. Additionally, the number of undulations per pumping period and overall pumping duration increased with temperature, suggesting a more intense irrigation. These findings suggest that rising temperatures may enhance chironomid-driven irrigation in tropical environments, affecting nutrient release and altering bacterial communities, with potential effects on organic matter decomposition and freshwater biogeochemical cycles. Understanding these responses is critical for predicting how tropical freshwater ecosystems will adapt to climate change, particularly in regions experiencing rapid warming.