Streams provide subsidies to terrestrial food webs in the form of emergent insects. However, most of our understanding of stream-to-terrestrial subsidies is from perennial systems. Limited work in non-perennial streams suggests substantial insect biomass production can occur during flow periods, even in the relatively arid and low-diversity systems thus far studied. We are studying two highly diverse non-perennial streams in the Ozark Highlands, a humid continental region of the central USA, to assess patterns of emergence and lateral flight. One stream contains fish, and the other is fishless. Given an expectation of faster development time and smaller adult size in streams with fish predators, we are also addressing these potential life-history differences and their influence on timing and biomass of subsidies. We are collecting emergent insects during two-week periods in each of the four key seasons of non-perennial streams: rewetting (November samples), full-flow (February and April samples), pooling (June), and dry (September). We run 6 emergence traps per stream during each sample period to characterize the diversity of emergent Ephemeroptera, Plecoptera, and Trichoptera, and a few Diptera taxa. We concurrently run 6 Malaise traps placed parallel to and within 1-2 meters of streams to estimate proportion of emerged insects undertaking lateral flight. We will also estimate subsidies as insect biomass produced per unit length of the streams. At time of writing, we have data from the rewetting season, with traps run 2-4 weeks after the return of surface water. As expected at this early stage, total abundance was low, but small-bodied Plecoptera and Trichoptera were already present in both trap types and were more abundant at the fishless stream. We anticipate increasing diversity and biomass as the flow season progresses and air temperatures rise. Studies highlighting the connectivity of small non-perennial streams to the watershed are important to draw attention to these underappreciated and under protected systems.