Headwater streams are important cold-water refugia and preserving them will become increasingly important as temperatures increase. This project was initiated over 10 years ago to encourage local landowners to contribute to the protection of identified refugia streams and catchments near Francestown, New Hampshire. As a result, large reaches of these headwater streams are now under conservation easement. Our goal is to continue to characterize this network of headwater streams in southern New Hampshire as environmental conditions change due to climate change and other anthropogenic stressors including habitat destruction/fragmentation (logging, dams), pollution (run-off), and invasive species. We will present results from monitoring temperature and water level fluctuations using water loggers and adapting methods leveraging machine learning and photo imaging using wildlife cameras in >10 headwater streams to characterize changes in temperature regimes and water levels. Additionally, we are adapting and developing environmental DNA protocols to observe the impact the impact of environmental change on two groups of bioindicator species, brook trout, a cold-water fish. Our data show small-scale variation in temperature regimes due to differences in their hydrology, geomorphology, and riparian coverage indicating differences in their suitability as climate refugia. Additionally, early trends indicate increasingly large fluctuations in water level as patterns of snow catchment and precipitation become more erratic from year to year. Though changes in temperature and precipitation patterns will inevitably impact the biological communities inhabiting the headwater streams long-term, currently we observe continued use of these critical habitats by brook trout and sensitive macroinvertebrate species.