Anthropogenic climate change and infectious diseases are leading causes of amphibian population decline around the world. Changes in precipitation and salinity have reduced habitat availability and altered physiological performances for amphibian survival. The salt refugee hypothesis however, is a proposed explanation that amphibians have stronger abilities to defend against pathogens in higher saline environments. Along the coast of California, Monterey County is a known breeding ground for endangered amphibian species. To increase conservation where sensitive populations reside, the prevalence and intensity of Batrachochytrium dendrobatidis (Bd) and the composition of skin microbial communities will be evaluated within the Pacific Tree Frog (Pseudacris regilla). We hypothesize amphibians in higher salinity concentrations will have lower Bd rates and more bacterial diversity. Fifteen skin swab samples were collected at 10 sites in the Moro Cojo Slough State Marine Reserve across a salinity gradient (0.5 ppt- 30 ppt). DNA was extracted for the molecular quantification of Bd and the skin microbiome. Bd prevalence and intensity will be assessed with quantitative PCR, while bacterial community composition will be evaluated by amplifying the V4-V5 region of the 16S rRNA gene using the Illumina Miseq platform. Statistical analyses, including Adonis method, Kruskal-Wallis test, and PERMANOVA test, will evaluate the interaction between Bd presence/absence, salinity concentrations, and microbial diversity. Spearman rank correlation and Mantel test will explore correlations between Bd infection intensity, salinity concentrations, and bacterial community structure. These results will provide valuable insight for amphibian conservation efforts where environmental conditions continue to change.