The Brazilian oil spill that occurred between 2019 and 2020 was the largest spill event in Brazil, with over 5300 tons of oil released along the northeast coastline. Polycyclic aromatic hydrocarbons (PAHs) present in the oil were shown to induce toxicity to early life stage fish following exposure, although the mechanisms in which these toxic responses are exhibited are not well understood. To better characterize these effects across different levels of biological organization, zebrafish (Danio rerio) were embryonically exposed to water accommodated fractions (WAFs) of oil containing 33 total PAHs (tPAH) at concentrations (2.25 and 17.94 µg/L tPAH) measured following the spill. Transcriptomic profiling, morphological assessment, histopathology, and behavioral assays were conducted in larvae that were 168 hours post fertilization (hpf). The top enriched gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were involved in phototransduction, visual perception, gas transport, and drug metabolism in oil-exposed larvae. Larvae had significant reductions in swim bladder diameter and eye area, which corresponded to reduced diameters in the outer plexiform and retinal pigment epithelial layers in oil-exposed larvae. To help anchor these molecular, morphological, and histological endpoints, behavioral assays involved in visually mediated responses and swimming activity will be assessed. This data suggests that the concentrations of PAHs in the oil spilled off the coast of Brazil has the potential to dysregulate proper swim bladder and eye development, which are important for the recruitment and survival of early life stage fish.