Prior examination of National Ecological Observatory Network (NEON) data found that benthic insect communities are largely stable through time, consistent with the loose equilibrium hypothesis. This study extends the previous analysis by searching for environmental effects of insect community structure. Time-series of six environmental variables – discharge, water temperature, dissolved oxygen, specific conductivity, nitrate, and turbidity – were downloaded for each of 20 NEON streams, then compared to time-series of the insect community data. Specifically, we: (i) calculated the distance between each insect community sample and the centroid of the respective ordination plot (non-metric multidimensional scaling); (ii) built a monthly time-series of the sample-to-centroid distances for insect community (i.e., NEON stream); (iii) compared the insect time-series with time-series (mean monthly averages) for each of the six environmental variables; and (iv) used Pearson correlation coefficients to assess the strength of association between insect and environmental time-series. Cross-correlation was also used to test for lagged or delayed environmental effects because insect community structure at a given moment may reflect antecedent, rather than contemporary environmental conditions. Time lags ranging from 0-6 months were simulated by shifting the insect time-series backwards, then recalculating correlation coefficients. Contemporary environmental conditions showed the strongest correlation in only 20 of 120 comparisons (6 variables × 20 streams). In most comparisons, the strongest correlation occurred at a lag of 4-6 months. This project highlights the potential importance of antecedent environmental effects and demonstrates the flexibility of NEON data.