ARAM (Antilles Rapid Assessment Method) is the product of a collaborative effort between the US Army Corps of Engineers and PR Department of Natural and Environmental Resources to establish a standardized wetland condition assessment tool for Puerto Rico and the U.S. Virgin Islands. Following the ARAM a-priori categorization, we selected 6 palustrine locations, 3 forested and 3 emergent wetlands, as representatives of “least-disturbed” conditions. A second group of palustrine wetlands (6) was chosen to represent “impaired” sites. From 2020 to 2023, we evaluated the environmental conditions of these wetlands. Biological indicators included a diatom-based Pollution Tolerance Index (PTI) [calculated in five different versions] and the Genera-based Index (GDI), as well as two macroinvertebrate-based indexes: Biological Monitoring Water Party Index (BMWP-PR) and Family-Based Index (FBI-PR), both as modified for Puerto Rico by Gutiérrez and Ramírez. Additional ecological indicators included soil metrics [i.e., total organic carbon (TOC), gravimetric water content (GWC), and permanganate oxidizable carbon (POXC)], among others. hydroperiod. Three diatom-based metrics [GDI and two versions of PTI] produced a classification of Puerto Rican palustrine wetlands comparable to that proposed by the ARAM study. According to the BMWP-PR scores, ten out of the twelve wetlands behaved as predicted by the ARAM-classification. There was a significant difference in the means of the BMWP-PR scores according to wetland type (forested vs. emergent), but not environmental condition (impaired vs. least-impaired). Meanwhile, there was a significant difference in the means of the FBI-PR scores according to environmental condition, but not wetland type. However, the best attainable water quality with FBI-PR for reference wetlands was “good”. Diatom-based PTI showed a negative strong correlation with TOC and POXC, while there was a negative correlation between FBI-PR with GWC. Thus, a longer permanence of the water (as evidenced by a concomitant higher water saturation of the soils) seem to translate into a more suitable habitat for sensitive species.