Larvae of midges (Diptera: Chironomidae) occur in diverse communities, are widely distributed and are abundant in many freshwater habitats. Chironomid larvae were collected by substrate pick sampling and multi-habitat kick sampling from streams in the Eastern Great Lakes Lowland (EGLL) and Erie Drift Plain (EDP) Ecoregions in northeastern Ohio. Two streams were sampled in each county between March 15th and April 15th. Mouthparts and other morphological structures of midge larvae were mounted to slides using CMC-1019, examined under a compound microscope and identified to the lowest taxonomic level (preferably species). EstimateS (Colwell 2013) was used to develop rarefaction and extrapolation curves for midge species richness using Chao 1 and Chao 2 estimators. The EstimateS indicated the data were a reasonably accurate representation of midge species richness for Ohio's EGLL and EDP and further sampling is not required. The distributions of the midge taxa in northeastern Ohio were mapped. Midge taxa collected from substrate pick sampling differed from that collected by multi-habitat kick sampling. Rare species included Cricotopus (I.) trifasciatus, Orthocladius (O.) dorenus, and Psectrocladius (Ps.) cf. octomaculatus. The most common taxa were Orthocladius (O.) sp., Tvetenia paucunca, Orthocladius (O.) robacki/obumbratus and Thienemanniella xena.