Pharmaceutical pollutants in freshwater ecosystems are an emerging environmental concern due to their persistence and potential to disrupt aquatic life. Ibuprofen, a widely used over-the-counter, non-steroidal anti-inflammatory drug, is becoming increasingly prevalent among these pollutants. Conventional water treatment processes fail to remove Ibuprofen effectively. Humans secrete 0.1g of Ibuprofen in urine after digestion. Due to its poor solubility, Ibuprofen binds to organic materials, accumulates, and persists in freshwater ecosystems. This study evaluated the locomotion behavioral responses of Xiphocaris elongata to varying doses of Ibuprofen. Specimens of Xiphocaris elongata were exposed to four doses of Ibuprofen (200mg, 400mg, 600mg, 800mg); the exposure tests were recorded and analyzed with LoliTrack. Behavioral responses were measured as percentages of active and inactive states over a 72-hours exposure period. At control treatment (0mg ), active behavior was 45% with no mortality; at 200mg, activity decreased slightly to 39% and no deaths. Notable effects were seen at 400mg, where active behavior dropped to 29% and the mortality rate reached 25%. At 600mg and 800mg doses led to further reductions in activity (36% and 12%, respectively), accompanied by mortality rates of 25% and 60%. Findings emphasize the ecological risks associated with Ibuprofen pollution, as even minimal concentrations can affect the behavior and survival of Xiphocaris elongata. This highlights the urgent need for improved management of pharmaceutical contaminants. Further studies should explore chronic and cumulative exposure impacts, integrating physiological studies, removal alternatives, and ecosystem-level analyses to gain a better understanding of the long-term consequences.