In the Caribbean, watersheds have been experiencing degradation of ridge to reef ecosystems due to more frequent extreme events of precipitation. These events have the potential of modifying the hydrological cycle by triggering higher rates of runoff, especially on those landscapes with poor water infiltration capacity. Water replenishment is a stewardship strategy oriented to increase the soil’s percolation capacity and reduce surface runoff that causes deterioration in watershed ecosystems. The Nature Conservancy (TNC) is implementing a volumetric water benefit accounting methodology created by the World Resources Institute, to quantify in cubic meters the reduction of runoff resulting from interventions of forest conservation and vegetation restoration. In 2024, TNC started its first water replenishment project in Puerto Rico, in the watersheds of the rivers Guayanés and Humacao. Land and vegetation coverage were mapped for both watersheds using optimal high-resolution satellite scenes and object-based image analysis, resulting in ten land-use categories. Then, areas with higher capacity to achieve water volumetric benefits were identified, considering factors such as vegetation cover and soil type. With the priority areas selected, the volumetric benefit expected from the implementation of activities intended to increase the vegetation cover was calculated. It was estimated that implementing practices like agreements to avoid land use alterations and replacement of introduced vegetation with native and endemic species in 675 hectares of land, could reduce 326 cubic meters of runoff. Also, restoring the vegetation cover by implementing agroforestry practices, reforestation, and sustainable livestock farming in 435 hectares of agricultural lands, could reduce 185 cubic meters of runoff. The estimated volumetric benefit represents avoided water-loss, additional water available for the other elements of the water cycle, and therefore, a healthier watershed system.