Salinization is an emerging concern that disrupts the structure and function of stream ecosystems, with degraded water quality potentially displacing sensitive biota and triggering ecological stresses that harm benthic invertebrate assemblages. These communities play a critical role in maintaining ecosystem balance, and their response to salinization can disrupt the food web stability. We sought to examine if and how salinization affects the diversity and composition of benthic invertebrates in low-disturbed streams, and how these impacts vary across six monitoring sites within the continental US. Sites from the National Ecological Observation Network (NEON) were selected from three landscapes (i.e., forested, agricultural, and mixed) partitioned by core and gradient environments. For each site, we retrieved records for conductivity, major ions (e.g., Cl-, Na+, Ca+, Mg+), stream flow, and benthic invertebrate composition (i.e., species richness and abundance) to assess community structure differences based on salinization impacts. We expect potential shifts towards salt-tolerant species (e.g., chironomids) in areas with greater human influence and altered land use. Higher stream flow in gradient areas may affect major ion concentrations depending on the surrounding landscape. Agricultural and mixed land uses are likely to experience the enrichment of salt ions, whereas in forested regions salts may be diluted in response to stream flow increases. All these findings could explain the complexity of salinization, stream flow, and benthic invertebrate communities to improve management practices and further salinization mitigation efforts across diverse landscapes.