Lymantria dispar dispar (European spongy moth) is a destructive folivore that has been introduced to the eastern United States. Mating disruption via introduction of the pheromone disparlure is the most common method of control used against low density populations by the National Slow the Spread Program (STS). SPLAT GM-Organic is currently the only disparlure formulation used in mating disruption treatments, but despite its prevalence, its impact on freshwater ecosystems is unknown. When introduced to water, the waxes that make up the majority of the SPLAT GM-Organic treatment partially dissolve and create a film on the surface of the water. While being biodegradable, these waxes are persistent and hydrophobic. We predict that the SPLAT treatment may disrupt these small bodies of water by reducing oxygen diffusion and altering food availability to aquatic macroinvertebrates. Our aim is to identify the short and long-term effects of the aerial application of SPLAT GM-Organic on aquatic organisms. We have devised a study forecasted to end in May 2027 and have identified 5 streams and 10 reaches in the Slate River Watershed. Five of these reaches are upstream of the aerially treated plots and will serve as the control. The baseline pretreatment data consists of 5 replicate Surber samples (250 um mesh) per reach. We sampled in summer and autumn prior to SPLAT spraying (June and October 2024). The pheromone treatments will occur in June 2025. For the short-term analysis, we will sample 24-48 hours after the pheromone treatments, two months after the treatment application, and again in mid-October. For longer term analysis, we will sample in early June, Late July, and mid-October, 2026. Our results will either demonstrate the safety of the current SPLAT GM-Organic treatment method or be used to develop more responsible applications.