The St. Louis River estuary has been subject to industrial contamination, overfishing and habitat degradation. Due to these factors the estuary has been designated an area of concern and has undergone major remediation and habitat restoration efforts. Lake Sturgeon (Acipenser fulvescens) historically have been extirpated from the St. Louis River Estuary and restoration and management of this species population has been ongoing. To determine the effectiveness of these restoration efforts, population and genetic surveys have been conducted to determine Lake Sturgeon status. Sex ratios of Lake Sturgeon populations are hard to determine in these surveys, due to Lake Sturgeon not reaching sexual maturity until around 15-25 years old. Recently a female-specific locus has been discovered in Lake Sturgeon allowing for a qPCR-melt curve assay to molecularly sex Lake Sturgeon using non-invasive fin clips. During the 2023 and 2024 field seasons, the Minnesota Department of Natural Resources and 1854 Treaty Authority collected fin clips from 302 sturgeon from the St. Louis River estuary. Currently the molecular sexing assay is being carried out to determine the sex ratio of this population. This is the first time this technique is being used in the St. Louis River estuary, and this work will aid in population models and management decisions for the area of concern. The views expressed in this presentation are those of the author(s) and do not necessarily represent the views or policies of the U.S. Environmental Protection Agency.