The Edwards Aquifer spans around 3,600 square miles across central Texas, USA and is the primary water source for San Antonio metropolitan area. Northern San Antonio is growing in development and is located directly above the recharge zone of the aquifer. Due to its proximity, this area contributes to groundwater vulnerability partially dependent on flow paths and hydrogeological features. Groundwater vulnerability may then impact the diversity of substrates available for biodegradation, such as sulfate, iron, and CH4 which will dictate microbial presence in groundwater and dominant metabolic pathways involved in biogeochemical cycling. This project aims to determine the spatial, seasonal, and metabolic variation of dominant microbial taxa within groundwater environments of the Trinity and Edwards Aquifer. Approximately 25-75 liters of groundwater (depending on turbidity of groundwater sampled) were collected from 7 groundwater wells across the Trinity Aquifer Contributing Zone and the Edwards Aquifer Recharge Zone during 4 sampling periods in 2024 to capture seasonal variation in subsurface flow and resultant water quality. Genomic DNA was extracted from collected water filters and shotgun metagenomic sequencing (Nanopore MINion long-read sequencing) is currently in progress. This will allow identification of specific gene prevalence of biogeochemical cycles, to determine environmentally driven microbial metabolism. Preliminary data from the first spring sampling period indicates groundwater wells in the Trinity Aquifer Contributing Zone have high total coliforms (MPN per 100 mL) likely dictated by surface conditions after precipitation events linking surface-aquifer interactions. These data will allow for a greater understanding between hydrogeological characteristics and microbial metabolic change which likely contribute to groundwater quality in the Edwards Aquifer system.