The Everglades crayfish, Procambarus alleni is a short-lived crayfish that lives in seasonal subtropical wetlands in Florida and is an important resource for nesting wading birds. The hydroperiod limitations of the populations of P. alleni and the mechanisms by which water depth variation produces high biomasses are not understood, but past studies concluded populations were stressed when hydroperiods were < 7 months. To look for patterns consistent with population limitation by shortened hydroperiods we sampled crayfish in six seasonal wetlands (i.e., 1 ha marl prairies) reflecting a hydroperiod gradient; three wetlands of shorter average hydroperiods (3-5 mo.)and three with longer hydroperiods (7- 9 mo.). Crayfish densities were quantified with replicated 1-m2 throw traps for three seasons each year from 2022-2024 whenever water depths exceeded 5 cm. To test for differences in suitability for individual growth, we conducted an in situ enclosure experiment to quantify summer growth rates of juvenile crayfish across the same wetlands. Results from the throw-trap sampling indicated that populations were juvenile-dominated with the highest densities of small juveniles timed to the summer re-flooding. Because longer-hydroperiod wetlands were flooded longer they supported more aboveground crayfish biomass on average. But when all wetlands were simultaneously flooded the biomass was only significantly (p< 0.5) greater in the longer hydroperiod wetlands in the early wet season sample of July 2024 and not in late wet season samples (Oct 2023, 2024). Results from the in situ growth experiment indicated no significant differences in juvenile crayfish growth among sites. We have little evidence, thus far, for population limitation by hydrologic stress limiting P. alleni. Nevertheless, short hydroperiods will reduce crayfish availability for foraging wading birds in the dry season because the wetlands consistently dry out and crayfish retreat belowground before wading birds initiate their nesting colonies.