Species interactions can have strong effects on communities. For example, non-consumptive effects of stream fish on invertebrate prey include life-history and behavioral responses at the population level that, in combination, affect prey community structure. Most of our understanding of these effects comes from perennial streams, with limited understanding in non-perennial streams. We are studying diverse non-perennial streams of the Ozark Highlands (central USA) to assess whether invertebrate communities respond to fish similarly to predictions from perennial streams. We collect data from two physically similar streams, both of which typically have surface flow from approximately November to May. One connects to a shared mainstem during high-flow events, which allows fish access. The other is continually fishless. We collected 4 replicate Hess samples in an exposed bedrock section and 4 in an alluvial section of both streams (N=16) in mid-February, a time point well into the flow season when benthic communities are well-developed, and we repeated this sampling in 2 different years to assess inter-annual variability. Preliminary data from the first of the two years indicate greater invertebrate diversity in the fishless stream but greater abundance in the fish stream. In both streams, abundance was greater on alluvial than bedrock substrate. These patterns are pronounced in Ephemeroptera and support a hypothesis that many mayflies have behavioral and life-history adaptations allowing them to thrive in the presence of fish, despite being common prey. With climate change, many perennial streams are becoming non-perennial, so it is important to understand ecological processes in non-perennial streams.