Environmental DNA (eDNA) analysis, the collection and identification of genetic traces that organisms shed into their surroundings in the form of sloughed cells and other wastes, represents one of the most rapidly expanding tools for the study and management of biodiversity in freshwater systems and beyond. To date, most eDNA applications have collected samples in the form of filtered water or bulk sediments; however, several recent studies have successfully collected and identified terrestrial plant, invertebrate, and vertebrate eDNA via airborne sampling. Therefore, we conducted laboratory and field experiments to determine whether eDNA from freshwater species occurs within airborne eDNA. We first asked whether eDNA from the zebrafish (Danio rerio) could be collected through airborne sampling in an ecotoxicology lab housing large experimental populations of the model organism. In addition to proof-of-concept, we explored the effect of sampling duration (5, 10, or 20 minutes or overnight) and sample collection method (passive or active) on eDNA detection. Next, to explore airborne aquatic eDNA sampling under natural conditions, we collected paired water and air samples from the shore of Buffalo Springs Lake near Lubbock, Texas, USA, targeting striped bass (Morone saxatilis) eDNA. eDNA in both laboratory and field experiments was quantified using species-specific quantitative PCR. We successfully detected zebrafish eDNA in our laboratory experiment; however, eDNA appeared to be both heterogenous and rare in the laboratory air, with no clear relationship to sampling duration or collection method. Analysis of field-collected samples remains underway. Overall, the successful detection of aquatic eDNA from the air provides an exciting proof-of-concept and expands the horizons for eDNA analysis in freshwater ecosystems. As with eDNA applications in other systems, future studies should learn more about the ecology of airborne eDNA- its origin, state, transport, and fate- to better understand possibilities, strengths, and weaknesses of airborne eDNA collection from aquatic organisms.