While previous studies have explored the impact of behavior and life history on environmental DNA (eDNA), little research has been conducted on the impact of molting on eDNA detectability and particle size for aquatic arthropods like crayfish. To address this knowledge gap, we conducted a controlled, laboratory study investigating the effect of molting on eDNA detectability in the marbled crayfish (Procambarus virginalis), an obligate parthenogenetic species with high invasive potential. We collected water samples from aquariums at pre- and post-molt time intervals and filtered our samples using sequential filtration from large to small filter pore sizes (5.0 μm, 1.0 μm, and 0.2 μm) to assess eDNA detectability across a range of particle sizes. We expected eDNA detectability to increase immediately after molting, and then decline post-molt to an equilibrium between eDNA generation and degradation. Contrary to our hypothesis, we found a weak negative or non-existent effect of molting on eDNA detectability suggesting that the post-molt period does not enhance detection rates. Instead, time spent in aquariums had a strong positive effect on eDNA presence, indicating a gradual accumulation of genetic material. Additionally, eDNA detectability was highest at the largest filter pore size (5.0 µm) regardless of molt status, suggesting that crayfish eDNA is predominantly found in larger cellular aggregates. These findings challenge the assumption that molting increases eDNA release and highlight the importance of other factors that affect eDNA detection. Given that molting had a weak effect on eDNA detectability for our study crayfish, we suggest that researchers align their sampling efforts with other seasonal life history events, like reproduction or egg extrusion, that have been demonstrated to improve eDNA detectability. These findings contribute to refining eDNA surveillance strategies, particularly for invasive crayfish species, and emphasize the complexity of eDNA dynamics in aquatic ecosystems.